{"product_id":"crispr-lbcas12a","title":"CRISPR LbCas12a","description":"\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003e Overview\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eTriAltus’ LbCas12a protein is a highly pure, tag-free CRISPR Type V-A nuclease optimized for genome editing and CRISPR diagnostic workflows. Derived from \u003c\/span\u003e\u003cem\u003eLachnospiraceae\u003c\/em\u003e\u003cspan\u003e\u003cem\u003e bacterium\u003c\/em\u003e and expressed in\u003cem\u003e \u003c\/em\u003e\u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e, LbCas12a recognizes TTTV PAM sequences and generates staggered 5’ overhangs, enabling precise DNA target engagement and multiplex editing capabilities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e LbCas12a nuclease, CRISPR Cas12a protein, Cpf1 nuclease, Lachnospiraceae Cas12a, Cas12a CRISPR nuclease\u003c\/span\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2 dir=\"ltr\"\u003eWhy Purity and Activity Matter for Cas12a\u003c\/h2\u003e\n\u003cp data-start=\"216\" data-end=\"1024\"\u003eCas12a (Cpf1) differs from Cas9 in both mechanism and workflow, placing added importance on enzyme purity and consistent activity for reproducible results. Cas12a relies on a single crRNA (without a tracrRNA), recognizes TTTV PAM sequences, and introduces staggered DNA cuts, making RNP assembly and cleavage efficiency particularly sensitive to inactive protein populations or impurities. In multiplex genome editing and CRISPR diagnostic workflows—where Cas12a’s collateral cleavage behavior is often leveraged—protein quality can directly influence signal clarity, background activity, and experiment-to-experiment consistency. High-purity, well-characterized Cas12a supports reliable crRNA binding and predictable cleavage performance, enabling more consistent results across diverse CRISPR applications.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2 dir=\"ltr\"\u003eBulk Supply \u0026amp; Program Support\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003eTriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO 13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. \u003ca href=\"https:\/\/trialtusbioscience.com\/contact\" title=\"discuss bulk supply and program requirements\"\u003e\u003cstrong\u003eContact TriAltus\u003c\/strong\u003e\u003c\/a\u003e to discuss volume, documentation, packaging, and supply planning.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2 style=\"text-align: left;\"\u003e\u003cspan class=\"qowt-stl-IntenseEmphasis\"\u003eKey Benefits\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"text-align: left;\"\u003eTag-free LbCas12a for reduced non-specific interactions\u003c\/li\u003e\n\u003cli style=\"text-align: left;\"\u003eClass 2, Type V-A CRISPR nuclease with precise PAM targeting\u003c\/li\u003e\n\u003cli style=\"text-align: left;\"\u003eDual nuclear localization signals (NLS) enhance nuclear delivery\u003c\/li\u003e\n\u003cli style=\"text-align: left;\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eIdeal for multiplex editing, sensitive detection, and diagnostics workflows\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2 style=\"text-align: left;\" id=\"E1576\"\u003e\u003cstrong\u003e\u003cspan class=\"qowt-stl-IntenseEmphasis\" id=\"E1577\"\u003eSpecifications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003ePURITY | \u003c\/b\u003e\u0026gt;90%\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eENZYME SOURCE | \u003c\/b\u003e\u003cem\u003eLachnospiraceae bacterium \u003c\/em\u003eCas12a is expressed in \u003cem\u003eE. coli\u003c\/em\u003e with two C-terminal nuclear localization signals (NLS) and is purified with our CLīM technology.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eSTORAGE\/SHIPPING CONCENTRATION | \u003c\/b\u003e~ 5 mg\/mL\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eSTORAGE BUFFER | \u003c\/b\u003e10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eRECOMMENDED STORAGE CONDITIONS | \u003c\/b\u003e-80°C\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eEXPIRATION | \u003c\/b\u003e6 months from receipt when stored as directed. Avoid repeated freeze\/thaw cycles. \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProduced under ISO 13485–compliant processes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0422\/6590\/3256\/files\/LbCas12a_Data_Sheet_v1.0.pdf?v=1765811784\" title=\"LbCas12a Data Sheet\"\u003eLbCas12a Data Sheet\u003c\/a\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eSKU List\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e100 µg: 40-1120 | \u003c\/span\u003e\u003cspan\u003e500 µg: 40-1125 | \u003c\/span\u003e\u003cspan\u003e1 mg: 40-1130 | Bulk: Inquire\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--themify_builder_static--\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eLbCas12a supports diverse CRISPR workflows:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eCRISPR–Cas12a RNP Genome Editing\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e LbCas12a forms stable RNP complexes with crRNA alone, permitting precise genome editing at TTTV PAM sites and enabling simultaneous editing of multiple targets in a single experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eCRISPR Diagnostics \u0026amp; Detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eBeyond gene editing, Cas12a’s collateral ssDNA cleavage activity underlies CRISPR-based detection strategies, including sensitive nucleic acid assays and point-of-care diagnostics.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eIn Vitro Cleavage \u0026amp; Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eThis protein is suitable for in vitro DNA cleavage and screening workflows, offering consistent performance for method development and comparative analyses.\u003c\/span\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eLearn More\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-start=\"176\" data-end=\"369\"\u003eLearn how TriAltus achieves high purity using \u003ca href=\"https:\/\/trialtusbioscience.com\/clim-technology\" title=\"CLīM™ one-step protein purification technology for high-purity recombinant proteins\"\u003e\u003cstrong data-start=\"222\" data-end=\"253\"\u003eone-step CLīM™ purification\u003c\/strong\u003e\u003c\/a\u003e, a high-affinity system designed to streamline recombinant protein production while maintaining consistent quality.\u003c\/li\u003e\n\u003cli data-start=\"373\" data-end=\"537\"\u003eExplore\u003cstrong data-start=\"381\" data-end=\"420\"\u003e \u003ca href=\"https:\/\/trialtusbioscience.com\/publications\" title=\"Peer-reviewed publications describing CLīM™ purification and protein production methods\"\u003epeer-reviewed publications\u003c\/a\u003e\u003c\/strong\u003e describing the underlying purification platform and protein production methods used across TriAltus enzyme products.\u003c\/li\u003e\n\u003cli data-start=\"541\" data-end=\"672\"\u003eFor related CRISPR enzymes, view the \u003ca href=\"https:\/\/trialtusbioscience.com\/products\/purified-aapcas12b-protein\" title=\"Highly pure AapCas12b Protein for CRISPR editing\"\u003e\u003cstrong data-start=\"578\" data-end=\"604\"\u003eAapCas12b Protein page\u003c\/strong\u003e\u003c\/a\u003e to compare alternative Cas12 family nucleases and workflow options.\u003c\/li\u003e\n\u003cli data-start=\"601\" data-end=\"734\"\u003e\n\u003cp data-start=\"603\" data-end=\"734\"\u003eTo compare nuclease selection across CRISPR workflows, see the \u003ca href=\"https:\/\/trialtusbioscience.com\/products\/purified-crispr-cas9-protein\" title=\"Highly pure Cas9 Protein for CRISPR editing\"\u003e\u003cstrong data-start=\"801\" data-end=\"833\"\u003ePurified CRISPR-Cas9 Protein\u003c\/strong\u003e\u003c\/a\u003e page\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2 dir=\"ltr\"\u003e\u003cspan\u003eFAQs\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eIs LbCas12a tag-free?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eYes, the protein is supplied without affinity tags for streamlined RNP formation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eWhat guides does LbCas12a use?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eLbCas12a complexes with a single crRNA sequence, no tracrRNA required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eWhat PAM does LbCas12a recognize?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cspan\u003eTTTV (V = A\/G\/C) PAM sequences for DNA targeting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--\/themify_builder_static--\u003e\u003c\/p\u003e","brand":"trialtusbioscience","offers":[{"title":"100 µg (40-1120)","offer_id":48134318457088,"sku":"40-1120","price":150.0,"currency_code":"USD","in_stock":true},{"title":"500 µg (40-1125)","offer_id":48134318489856,"sku":"40-1125","price":600.0,"currency_code":"USD","in_stock":true},{"title":"1 mg (40-1130)","offer_id":48134318522624,"sku":"40-1130","price":1125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0422\/6590\/3256\/files\/LbCas12a_Product_Image_v4.jpg?v=1765815251","url":"https:\/\/trialtusbioscience.myshopify.com\/products\/crispr-lbcas12a","provider":"TriAltus Bioscience","version":"1.0","type":"link"}