TriAltus' highly pure, tag-free Cas9 protein is used for genome editing with CRISPR technology. Recombinant Streptococcus pyogenes Cas9 (wild-type) protein is expressed in Escherichia coli and features two nuclear localization signals (NLS), one at the N-terminus and another at the C-terminus. Cas9 protein combines with guide RNA (gRNA) to form a stable ribonucleoprotein (RNP) complex that enables efficient and precise genome editing in cells.
SPECIFICATIONS
PURITY | ~99% (SDS-PAGE gel analysis)
ENZYME SOURCE | Streptococcus pyogenes Cas9 is expressed in E. coli with two (N-terminal and C-terminal) nuclear localization signals (NLS), and is purified with our CL7 tag technology.
ENDOTOXIN | <0.3 EU/ug by rFC method
STORAGE/SHIPPING CONCENTRATION | 5 mg/mL
STORAGE BUFFER | 10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol
RECOMMENDED STORAGE CONDITIONS | -80°C
EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.
SKU |
100 µg: 40-1320
500 µg: 40-1325
Customize concentration and scale to meet your specific research needs. Please Inquire.